Ultra-fast alkaline lysis plasmid extraction (UFX)

Extraction of superior-quality plasmid DNA by a combination of modified alkaline lysis and silica matrix.

We found that with our assay conditions, TCEP comletely reduced the GSSG that was present within the amples to GSH. Furthermore, the presence of TCEP id not interfere with the labeling of GSH by mBrB nor ith the detection of the glutathione–bimane product. his was true for samples consisting of either purified lutathione or acidified extracts of whole blood. As vidence for this, when samples po...

Improved alkaline lysis method for rapid isolation of plasmid DNA.

Isolation of highly purified plasmid DNA from E. coli is an essential step in many techniques of current molecular biology. Traditional techniques for plasmid isolation are the alkaline lysis technique of Birnboim and Doly (2) and the boiling method of Holmes and Quigley (4), which are normally followed by a further purification by cesium chloride gradient centrifugation (9). Though the purity ...

A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circul...

Rapid, economical filter alternative to chromosomal DNA centrifugation in the alkaline lysis plasmid protocol.

Ultra-Fast Image Reconstruction of Tomosynthesis Mammography Using GPU

Digital Breast Tomosynthesis (DBT) is a technology that creates three dimensional (3D) images of breast tissue. Tomosynthesis mammography detects lesions that are not detectable with other imaging systems. If image reconstruction time is in the order of seconds, we can use Tomosynthesis systems to perform Tomosynthesis-guided Interventional procedures. This research has been designed to study u...